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KMID : 0903619940350020172
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Journal of the Korean Society for Horticultural Science
1994 Volume.35 No. 2 p.172 ~ p.177
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Micropropagation of Alstroemeria spp . through Rhizome Tip culture
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Abstract
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Rhizome tips of Alstroemeria spp. separated from mother plants were cultured in vitro on Murashige-Skoog medium supplemented with several growth regulators. Rhizome tips from green house grown plants were significantly contaminated even after surfacedisinfestation with 1% NaOCl (v/v) under in vitro condition. Among cytokinins, BA was most effective for shoot and rhizome multiplication, whereas kinetin and 2iP were not; optimal concentrations being 1.0-2.0 ㎎/ℓ BA. Combinations of BA with low levels of auxins were more favorable in rhizome multiplication than those of BA treatment alone; the media supplemented with 1.0-2.0 ㎎/ℓ BA and 0.3 ㎎/ℓ IAA or 0.2 ㎎/ℓ NAA were more effective for rhizome multiplication than other treatments tested. Comparing two types of explants, apical rhizomes produced more multiple rhizomes than lateral rhizomes. Lighting longer than 16 hours per day in 3,000 lux was effective for shoot and rhizome multiplication. Optimal subculture period to maintain organogenic capacity of rhizomes was every 3 weeks, but there were showed various differences in organogenesis among cultivars tested. The medium with 3.0 ㎎/ℓ IBA was effective for rooting.
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